Journal: Translational Cancer Research

Article Title: MIER3 suppresses the progression of non-small cell lung cancer by inhibiting Wnt/β-Catenin pathway and histone acetyltransferase activity

doi: 10.21037/tcr.2020.01.07

Figure Lengend Snippet: MIER3 down-regulates Wnt/β-catenin signaling. A549 and H460 cells were transfected with pcDNA3.1-vector or pcDNA3.1-MIER3 for 24 h. (A) The protein levels of β-catenin, Cyclin D1, c-Myc, and MMP7 were detected by Western blot. (B) Quantification of relative protein levels in A549. (C) Quantification of relative protein levels in H460. *, P<0.05 versus vector control. MIER3, mesoderm induction early response 1, family member 3.

Article Snippet: In this analysis, the following reactives (suppliers) were used: antibody to MIER3 (Abcam, Cambridge, MA, USA); antibodies to bax, BCL-2, cleaved caspase3, β-catenin, cyclin D1, c-Myc, MMP7 and p300 (Santa Cruz Biotechnology, CA, USA); antibodies to acetylated Histone3, acetylated Histone4, Histone3 and Histone4 (Cell Signaling Technology, Danvers, MA, USA); antibody to β-actin and HRP-conjugated secondary antibodies (SunShine Biotechnology, Nanjing, China).

Techniques: Transfection, Plasmid Preparation, Western Blot, Control

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: HER2 and APC Mutations Promote Altered Crypt-Villus Morphology and Marked Hyperplasia in the Intestinal Epithelium

doi: 10.1016/j.jcmgh.2021.04.012

Figure Lengend Snippet: HER2 V777L expression increases intermediate cells per villi in the small intestine. ( A ) Immunohistochemistry staining for lysozyme and PAS on intestinal villi. Scale bars are 100 μm. ( B ) Quantification of Paneth cell number per villus and ( C ) quantification of PAS-positive cells per villus. Eighty intestinal villi were measured; n = 4 per group. ( D ) Representative immunofluorescence staining of the proximal small intestine of Lgr5-Cre; HER2 V777L Tg and littermate control mice using MUC2 (red) and MMP7 (green) and merged images (with DAPI, blue). Yellow-colored cells indicate presence of intermediate cells with green and red colors merged. Scale bars are 100 μm. ( E ) Quantification of intermediate cells for the proximal small intestine. More than 50 villi were counted per mouse and n = 2 per group. ( F ) Transmission electron microscopy (TEM) representative images of goblet cells on the villi, Paneth cell at the base of the crypt, and intermediate cells near the crypt from Villin-Cre; HER2 V777L Tg mice. Scale bars are 2 μm.

Article Snippet: Primary antibodies used in the study include rabbit MUC2 (Santa Cruz Biotechnology, Dallas, TX; sc-15334), mouse MMP7 (R&D Systems, Minneapolis, MN; AF2967-SP), phospho-p44/42 MAPK (Erk1/2) (Thr202/204) (Cell Signaling Technology, Danvers, MA; #9101).

Techniques: Expressing, Immunohistochemistry, Staining, Immunofluorescence, Control, Transmission Assay, Electron Microscopy